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It is possible to allow the player to Learn Slash with a room type in the middle of a quest. However, most quest makers choose to either begin their quest with the Slash ability already available, or to forego it entirely.
With the addition of scripting, some players choose to create an item similar to the Tiger Scrolls that allows slashing. Whether or not Link can slash can be accessed with "Game->Generic[GEN_CANSLASH]", with 0 meaning not able and 1 meaning able.